Product Details
- PHERAstar FSX HTS Multimode Plate Reader with UV/VIS spectrometer and improved robotic integration
- Three integrated barcode readers streamline assays
- Simultaneous Dual Emission (SDE) detects assays with two emission wavelengths in one single measurement
- Three dedicated light sources, Decay Curve Monitoring, software-controlled top/bottom reading for assay flexibility
- Multimode shaking capabilities, on-board reagent injectors, PMTs optimized for different detection modes
- Detection modes: UV/vis absorbance spectra, Fluorescence intensity - incl. FRET, Luminescence (flash and glow) - incl. BRET, Fluorescence polarization, Time-resolved fluorescence, TR-FRET, AlphaScreen®/AlphaLISA®/AlphaPlexTM
- Measurement modes: Top and bottom reading, Endpoint and kinetic, Sequential multi-excitation, Sequential multi-emission, Simultaneous Dual Emission, Spectral scanning (absorbance), Real-time ratiometric measurements, Well scanning
- Microplate formats: Up to 3456-well plates, user-definable, LVis Plate with 16 low-volume microspots (2 μL)
- Light sources:
- High energy xenon flash lamp
- Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
- Dedicated laser for TRF and TR-FRET
- Detectors: Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes CCD spectrometer
- Wavelength selection:
- Optic Modules: up to six application-specific modules (includes all filters, dichroics, and etc. for an application)
- For top and bottom reading
- UV/vis absorbance spectrometer: full spectrum or up to 8 distinct wavelengths in < 1 sec / well
- Spectral range filters:
- 230 - 750 nm or 230 - 900 nm for FI, FP
- 230 - 750 nm for LUM
- 230 - 900 nm for TRF
- Spectral range Spectrometer: 220 - 1000 nm for ABS
- Sensitivity:
- FI (top): < 0.15 pM fluorescein (384sv black, 20 μL); < 0.5 pM fluorescein (1536 black, 8 μL)
- FI (bottom): < 1.0 pM fluorescein (384g black, 50 μL)
- FP: < 0.5 mP SD at 1 nM fluorescein (384sv black, 20 μL); < 1.5 mP SD at 1 nM fluorescein (1536 black, 8 μL)
- TRF with laser: < 5 fM europium (384, 80 μL); < 15 fM europium (1536, 8 μL)
- HTRF® (black and white microplates): Reader Control Kit (Eu) after 18h (384sv, 20 μL); Delta F > 1100 % (High Calibrator); Delta F > 25 % (Low Calibrator)
- LUM: < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL); Dynamic range: 9 decades
- AlphaScreen® with laser: < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)*
- ABS with spectrometer: Full spectrum captured in < 1 s / well; Selectable spectral resolution: 1, 2, 5, and 10 nm; OD range: 0 - 4 OD; Accuracy: < 1% at 2 OD; Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
- Read times:
- Flying mode (1 flash): 14s (384), 27s (1536)
- 10 flashes: 38s (384), 1 min 52 s (1536)
- 50 flashes: 1 min 29 s (384), 5 min 18 s (1536)
- Reagent injection:
- Up to 2 built-in reagent injectors
- Injection at measurement position (6 to 384-well)
- Individual injection volumes for each well (3 to 500 μL, optionally up to 2 mL)
- Variable injection speed up to 420 μL / s
- Up to four injection events per well
- Reagent back flushing
- Additional features of PHERAstar FSX HTS Plate Reader:
- Optical filters: Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
- Z-adjustment: Automatic focal height adjustment (0.1 mm resolution)
- Shaking: Linear, orbital, and double-orbital with user-definable time and speed
- Incubation: +5 °C above ambient up to 45 °C; (incubation chamber upper heating plate operates at 0.5ºC more than lower plate to prevent condensation build-up on sealer or lid)
- Robot compatible microplate carrier
- Software: Multi-user Reader Control and MARS data analysis software included (FDA 21 CFR Part 11 compliant)
- High performance module based optical system with barcode reader
- Four PMT measurement system, optimized for specific modes
- Rapid transport system provides fastest read times of PMT based microplate readers
- Standard Service Contract for one year including free software upgrades
- Optional Accessories: THERMOstar, LVis Plate, Stacker
- Upgrades: Speak or chat with a product specialist for information on detection modes, reagent injectors, etc.
Specs
• Type: Plate Reader
• Application: ELISA, Protein Assays, Nucleic Acid Assays
• Wavelength Range: 220 to 1000 nm
• Plate Configuration: 1536 Well
• Reader Mode: Multimode
• Manufactured by: BMG LabTech
• Manufacturer SKU: PHERAstar
• Unit of Measure: EA
• Weight, Product: 108.02 lb
• Dimensions, Product: 17.7" W x 20.07" D x 18.05" H
