Microplate Reader; Ensight™, Multimode, Perkin Elmer, 120 V

The EnSight™ Multimode Plate Reader features multiple data acquisition modalities that allow researchers to characterize several components of a single cell-signaling cascade in a single well. The fact that data are acquired and normalized on a per cell basis enable researchers to interrogate the dependence and magnitude of amplification that occurs as the signal travels downstream.

EnSight Well-Imaging Modes

The cell-image mode of the EnSight performs at high speed with an advanced sCMOS camera that features low signal-to-background noise, laser-based autofocus and solid-state LEDs for short illumination. This modality allows users to select fluorescence, Brightfield or digital phase-content imaging modes. All these features culminate in a well-imaging module that delivers greater physiological relevance to your analysis.

Well imaging can be used to normalize data based on an individual well's population. Traditional in vivo cell based assays required researchers to use a separate assay to quantify cell number for data normalization. The well imaging technology of the EnSight automatically makes a cell number measurement and normalizes data. This powerful feature provides laboratories with a significant return on investment by reducing the number of runs.

Runs multiplex assays sequentially in a 384-well plate:

• Single color image: 4 minutes
• Two-color image: 5 minutes
• Three-color image: 6.5 minutes (approximately)

The Corning® Epic® technology for labeled and label-free imaging allows cell-based and biochemical assays to run on one platform that delivers rich, unbiased data without interference. Label-free measurement allows multiple measurements on the same plate without affecting cell viability or the need for reagents. The first image shows a cell population measured with the label-free modality and luminescence units (LU) quantified. The same cell population was then measured via the ATPLITE® luminescence based cell viability assay. The second image clearly demonstrates that the label-free measurement modality has no effect on cell viability.

Benefit: Characterizes cellular signaling mechanisms completely and with more sensitivity; use of labeled and label-free technologies provide more physiologically relevant data from target identification and screening to hit confirmation.

Applications

• Cell-based assays: Cell toxicity, Neuron biology, Phenotypic assays, Primary and stem cells, Receptor biology, Response to any stimulus, Suspension cell culture
• Biochemical assays: Aggregation, Protein- protein interactions, Small molecule binding Alpha Technology Measurement Mode

Ideal for target-based, biochemical and cell-based assays, the powerful Alpha Technology measurement modality can be used to examine virtually any line of investigation traditionally measured via an ELISA assay.

Benefits

Provided AlphaLISA® and AlphaScreen® detection produce results in half the time of standard ELISA

Applications

High-throughput analysis of bio-therapeutics, epigenetics, protein-protein interactions, G-protein coupled receptor pathways, kinase cascades and many other intracellular interactions and signaling pathways